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KMID : 0613820220320080659
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Journal of Life Science
2022 Volume.32 No. 8 p.659 ~ p.665
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Molecular Characterization of the Nitrate Reductase Gene in Chlorella vulgaris PKVL7422 Isolated from Freshwater in Korea
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Abdellaoui Najib
Kim Min-Jeong
Choi Tae-Jin
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Abstract
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Chlorella vulgaris is an important freshwater alga that is widely used as a food source by humans and animals. Recently, Chlorella has received considerable attention with regard to its potential application in aquaculture and the production of biofuels, nutrients, and therapeutic proteins. Recently, our laboratory acquired a new strain of C. vulgaris, PKVL7422, characterized by fast growth, ease of culture, and cultivability under dark conditions. However, the genes involved in its nitrogen assimilation are unknown. In this work, we identified the nitrate reductase (NR) gene of C. vulgaris PKVL7422 using rapid amplification of cDNA ends and genome walking. The NR gene of C. vulgaris PKVL7422 is approximately 8 kb long and composed of 18 introns and 19 exons, which encode 877 amino acids. An alignment analysis of the NR gene showed that it possesses the five domains and several invariant residues found in plant NRs. These results provide new insight into the molecular organization of the NR gene in algae.
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KEYWORD
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Genome walking, nitrogen assimilation, phylogeny, RACE-PCR
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